Main

Fishman et al. (2001) build a genetic linkage map of an interspecific cross between M. guttatus and M.nasutus. At that time, the \(F_2\) mapping population used (N=526) were genotyped for 174 markers. Based on this study, the authors have found a genetic map of 2011-2096 cM Kosambi size, containing 174 marker loci distribuited over 14 linkage group.

Here we incorporate new genomic information onto the Fishman’s map in order to update the new loci information. By using a new data set of the same population with 418 markers, we founded

For more details about the procedures founded in this paper, also the strategies to build a genetic linkage map using OneMap R-package, please check our youtube video here[].

Results and Discussion

A total of 14 linkage groups were formed (Figure 1), which agrees to the haploid number of chromosomes. The genetic map drawn with MapChart is presented in Figure 2.

Figure 1. Heatmaps describing marker ordering over the 14 linkage groups of Mimulus guttatus.

Figure 2. MapChart drawing the 14 linkage groups and the loci information updated in this study.

Material and Methods

References

Broman, K. W., and S. Sen, 2009 A Guide to QTL Mapping with R/qtl, Vol. 46. Springer, New York.

Broman, K. W., H. Wu, S. Sen, and G. A. Churchill, 2003 R/qtl: QTL mapping in experimental crosses. Bioinformatics 19: 889–890.

Fishman, L.A., Kelly, J., Morgan, E., Willis, J.H., 2001 A genetic map in the Mimulus guttatus species complex reveals transmission ratio distortion due to heterospecific interactions. Genetics 159: 1701-1716.

Fishman, L. A., and J. H. Willis, 2001 Evidence for Dobzhansky-Muller Inteincompatibilities contributing to the sterility of hybrids between Mimulus guttatus and M. nasutus . Evolution 55: 1932–1942

Kosambi, D.D., 1944 The estimation of map distance from recombination values. Annuaire of Eugenetics 12:172-175.

Margarido, G.R., Souza, A.P, Garcia, A.A.F., 2007 OneMap: software for genetic mapping in outcrossing species. Hereditas 144:78-79.

Voorrips, R.E., 2002 MapChart: software for the graphical presentation of linkage maps and QTLs. Journal of Heredity.93: 77-78.

Wu, R., Ma, C.-X., Painter, I. and Zeng, Z.-B. (2002) Simultaneous maximum likelihood estimation of linkage and linkage phases in outcrossing species.Theoretical Population Biology 61: 349-363.